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Publications
35. Le, Q., Nguyen, V., Park, S.,
Recent advances in the engineering and
application of streptavidin-like molecules, Applied
Microbio & Biotech 2019 (doi:
10.1007/s00253-019-10036-5)
34. Zhou, F., Kroetsch, A., Nguyen, V.P., Huang,
X., Ogoke, O., Parashurama, N., Park,
S., High affinity antibody detection
with a bivalent circularized peptide containing
antibody binding domains, Biotechnol J [doi:
10.1002/biot.201800647] (Epub 2019)
33. Kroetsch, A., Qiao, C., Heavey, M., Guo, L.,
Shah, D.K., Park, S.,
Engineered pH dependent recycling antibodies
enhance elimination of Staphylococcal
enterotoxin B superantigen in mice, mAbs
11, 411-421 (2019)
32. Kroetsch, A., Chin, B., Nguyen, V., Gao, J.,
Park, S. Functional expression
of monomeric streptavidin and fusion proteins in
Escherichia coli: applications in flow cytometry
and ELISA, Applied Microbio & Biotech 102,
10079-10089 (2018)
31. S. H. Lee, C. Jin, E. Cai, P. Ge, Y.
Ishitsuka, K. W. Teng, A. A. de Thomaz, D. Nall,
M. Baday, O. Jeyifous, D. Demonte, C. M. Dundas,
S. Park, Delgado, J., W. N. Green, P.
R. Selvin. Super-resolution Imaging of Synaptic
and Extrasynaptic Pools of Glutamate Receptors
with Different-sized Fluorescent Probes, eLife
e27744 (2017)
30. Mann, J.K., Shen, J., Park, S.
Enhancement of muramyl dipeptide-dependent NOD2
activity by a self-derived peptide J Cell
Biochem 118:1227-1238 (2017)
29. Mann, J.K.(*), Demonte, D.(*), Dundas, C.M.,
Park, S., Cell labeling and
proximity dependent biotinylation with
engineered monomeric streptavidin, Technology
4, 1-7 (2016), DOI: 10.1142/S2339547816400057
28. Chamma, I., Letellier, M., Butler, C., Lim,
K.H., Gauthereau, I. Choquet, D., Sibarita,
J.B., Park, S., Sainlos, M.,
Thoumine, O., Mapping the dynamics and nanoscale
organization of synaptic adhesion proteins using
monomeric streptavidin, Nature Commun
7:10773 (2016) (DOI: 10.1038/ncomms 10773)
27. Demonte, D., Li, N., Park, S.,
Postsynthetic domain assembly with NpuDnaE
and SspDnaB split inteins. App
Biochem Biotech 177, 1137-51 (2015)
26. Kroetsch, A. and Park, S.
More than one way to skin a cat: in-situ
engineering of an antibody through
photo-conjugated C2 domain, Biotechnol J
10, 508-509 (2015)
25. Wang, H., Carrier, S., Park, S.,
Schultz, Z.D., Selective TERS Detection and
Imaging through Controlled Plasmonics, Faraday
Discussions 178:221-35 (2015)
24. Demonte, D., Dundas, C.M., Park, S.,
Expression and purification of soluble monomeric
streptavidin in E. coli. App Microbiol
& Biotech 98, 6285-6295 (2014)
23. Mann, J.K., Park, S.
Epitope specific binder design by yeast surface
display. Meth Mol Biol 1319:143-54
(2015)
22. Dundas, C.M., Demonte, D., Park, S.,
Streptavidin-biotin technology: improvements and
innovations in chemical and biological
applications. App Microbiol & Biotech
97:9343-9353 (2013)
21. Park, S., Mann, J.K., Li,
N., Targeted inhibitor design: lessons from
small molecule drug design, directed evolution,
and vaccine research. J SciMed Central CEPT
1:1004(2013). (read).
 
20. Demonte, D., Drake, E., Lim, K.H., Gulick,
A., Park, S., Structure based
engineering of streptavidin monomer with a
reduced biotin dissociation rate. Proteins:
Structure, Function, and Bioinformatics 81,
1621-1633 (2013)
 
19. Mann, J.K., Wood, J.F., Stephan, A.F.,
Tzanakakis, E.S., Ferkey, D.M., Park,
S. Epitope guided engineering of
monobody binders for in vivo inhibition of Erk-2
signaling. ACS Chem Biol 8, 608-616
(2013)
18. Lim, K.H., Huang, H., Pralle, A., and Park,
S. Stable, High-Affinity Streptavidin
Monomer for Protein Labeling and Monovalent
Biotin Detection. Biotechnology Bioeng
110, 57-67 (2013)
(Video abstract available on youtube.)
(Plasmids available at Addgene: pRSET-mSA,
pRSET-mSA-EGFP,
pDisplay-mSA-EGFP-TM,
pYD1-mSA.)
17. Lim, K.H., Huang, H., Pralle, A., and Park,
S. Engineered streptavidin monomer
and dimer with improved stability and function.
Biochemistry 50, 8682-8691 (2011)
16. Lim, K.H., Hwang, I. and Park, S.
Biotin-assisted folding of streptavidin on the
yeast surface. Biotechnol Prog 28,
276-283 (2011)
15. Hsu, C.K. and Park,
S. Computational and mutagenesis
studies of the streptavidin native dimer
interface. J Mol Graph Model 29,
295-308 (2010)
-Featured
as "Topical Perspectives"
14. Lim, K.H., Hsu, C.K., Park, S.
Flow cytometric analysis of genetic FRET
detectors containing variable substrate
sequences. Biotechnol Progress 26,
1765-1771 (2010)
13. Lim, K.H., Madabhushi, S.R. (*), Mann, J.
(*), Neelamegham, S., Park, S.
Disulfide trapping of protein complexes on the
yeast surface. Biotechnol Bioeng 106,
27-41 (2010). *Equal contributions.
-
Featured in Spotlight ("Getting STUCKED")
12. Sheldon Park and Jennifer
Cochran (Eds). Protein Engineering and
Design, CRC Press (2009)
11. Hwang, I. and Park, S.
Computational design of protein therapeutics. Drug
Disc Today: Tech 5, e43-48 (2008).
10. Szep,S.(*), Park, S.(*#),
Boder, E. T., Van Duyne, G., Saven, J. G.
Structural coupling between FKBP12 and buried
water. Proteins: Structure, Function,
and Bioinformatics 74, 603-11
(2009). (*) Equal contributions, (#)
Corresponding author.
9. Park, S., Xu, Y., Stowell,
X. F., Gai, F., Saven, J. G., Boder, E. T.
Limitatioins of yeast surface display in
engineering proteins of high thermostability. Protein
Eng Des Sel (PEDS) 19, 211-217 (2006).
8. Park, S. & Saven, J. G.
Simulation of pH-dependent edge strand
rearrangement in human b-2 microglobulin. Protein
Sci 15, 200-207 (2006).
7. Park, S. & Saven, J. G.
Statistical and molecular dynamics studies of
buried waters in globular proteins. Proteins
60, 450-463 (2005).
6. Park, S., Saven, J. G.
Computationally assisted protein design. Annu
Rep in Compu Chem, Volume 1, Chapter 18,
pp245- 253 (2005).
5. Park, S., Boder, E. T.,
Saven, J. G. Modulating the DNA affinity of
Elk-1 with computationally selected mutations.
J Mol Biol 348, 75-83 (2005).
4. Park, S., Kono, H., Wang,
W., Boder, E. T., Saven, J. G. Progress in the
development and application of computational
methods for probabilistic protein design. Comp
Chem Eng 29, 407 (2005).
3. Park, S., Fu, X., Wang,
W., Yang, X., Saven, J. G. Computational
protein design and discovery. Annu Rep
Prog Chem Sect C 100, 195-236 (2004).
2. Park, S., Yang, X.,
Saven, J. G. Advances in computational protein
design. Curr Opin Struct Biol 14,
487-494 (2004).
1. Park, S., Uesugi, M.,
Verdine, G. L. A second calcineurin binding
site on NFAT regulatory domain. Proc Natl
Acad Sci U S A 97, 7130-5 (2000).
Patent
Sheldon Park and Kok Hong Lim, Compositions
Comprising Monomeric Streptavidin and Methods
for Using Same, US Patent filed (Application no.
PCT/US10/32542)
 
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