Bioengineering Laboratory
Department of Chemical Engineering
906 Furnas Hall
State University of New York at Buffalo
Buffalo, NY 14260-4200
Phone: (716) 645-2911 ext. 2220
Fax : (716) 645-3822
E-mail: neel@eng.buffalo.edu
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Sriram Neelamegham Bioengineering Laboratory Department of Chemical Engineering 906 Furnas Hall State University of New York at Buffalo Buffalo, NY 14260-4200 Phone: (716) 645-2911 ext. 2220 Fax : (716) 645-3822 E-mail: neel@eng.buffalo.edu |
Associate Professor, State University of New York at Buffalo,
2003-present
Assistant Professor, State University of New York at Buffalo, 1997-2003
Research Associate, Baylor College
of Medicine, Houston, 1995- 1997.
Ph.D., Chemical/Biomedical Engineering, Rice
University, Houston, 1996.
B.Tech., Chemical Engineering, Indian Institute of Technology, Delhi,
India, 1991.
· RESEARCH OPENINGS
· Research Interests
· Publications
· Courses
· Supplemental
Data
Research Interests:
Research in our laboratory lies
in the field of Biomedical Engineering. The underlying theme is to
apply
novel bioengineering techniques and quantitative methodologies in
combination
with fundamental biological principles, to elucidate the parameters and
mechanisms that regulate leukocyte and platelet function. The
importance
of the studies stem from the fact that ailments of the blood involving
these cell types account for a sizable fraction of inflammatory and
thrombotic
disorders. The focus of our work is on three families of adhesion
molecules
and their ligands expressed on blood cells and the vascular
endothelium.
These molecules are members of the selectin, integrin and Ig gene
superfamily.
Ongoing projects in the laboratory also examine the effects of fluid
shear
on the structure and function of plasma protein, von Willebrand Factor.
Some of the emphasis of
our
laboratory is on developing experimental and theoretical
bioengineering/
biophysical approaches to determine the features that regulate
leukocyte
and platelet adhesion. In particular, we are interested in
understanding
how mechanical forces applied on vascular cells due to fluid flow
modulate
cellular and molecular function. Another research project in our
laboratory
examines the biology of leukocytes and platelets. Here, studies are
performed
to develop an understanding of the features that regulate the
time-dependent
changes in leukocyte and platelet function. In particular, we are
interested
in determining how changes in adhesion molecule expression, affinity,
topography
and intra-cellular signaling modulate cellular affinity. We are also
involved
in the development of novel therapeutic strategies to combat
inflammatory
diseases especially directed against the selectin family of adhesion
molecules.
Singh, I., Shankaran, H., Beauharnois, M.B.,
Xiao, Z., Alezxandridis, P., Neelamegham, S., "Solution structure of
human Von Willebrand factor studied using small angle neutron
scattering", J. Biol. Chem., 281(50):38266-275, 2006 [ABSTRACT].
Beauharnois, M.B., Neelamegham, S. and Matta,
K.L.,
"Quantitative measurement of selectin-ligand interactions: Assays to
identify
a sweet pill in a library of carbohydrates ", Glycobiology Protocols-
Methods
Mol Biol, 347:343-58, 2006 [ABSTRACT].
Chandrasekaran, E.V., Xue, J., Neelamegham, S., Matta, K.L., "The pattern of glycosyl- and sulfotransferase activities in cancer cell lines: a predictor of individual cancer-associated distinct carbohydrate structures for the structural identification of signature glycans", Carbohydrate Res. 341(8): 983-94, 2006 [ABSTRACT].
Xiao, Z., Goldsmith, H.L., McIntosh, F.A., Shankaran, H., Neelamegham, S., “P-selectin PSGL-1 bonds in neutrophil-platelet adhesion: Shear threshold and integrin-independent cell adhesion”, Biophys. J., 90(6):2221-34, 2006 [ABSTRACT].
Beauharnois, M.E., Lindquist, K.C., Marathe, D., Vanderslice, P., Xia, J., Matta, K.L., Neelamegham, S. “Affinity and Kinetics of Sialyl Lewis-X and Core-2 based Oligosaccharides Binding to L- and P-selectin ”, 44(27):9507-19, Biochemistry, 2005 [ABSTRACT].
Chandrasekaran, E.V. Xue, J., Xia, J., Chawda, R., Piskorz, C., Locke, R.D., Neelamegham, S., Matta, K.L. “Specificity analysis of sialyltransferases towards mucin core-2, Globo and related structures: Identification of the sialylation-sequence and the effects of sulfate, fucose, methyl and fluoro substituents of the carbohydrate chain in thebiosynthesis of selectin and siglec ligands, and novel sialylation by cloned alpha 2,3(O)sialyltransferase ”, 44(47):15619-35, Biochemistry, 2005 [ABSTRACT].
Liu, G., Swihart, M.T., Neelamegham, S., "Sensitivity, principal component and flux analysis applied to signal transduction: The case of epidermal growth factor mediated signaling", 21(7):1194-202, Bioinformatics, 2005 [ABSTRACT][Supplemental Data]
McDonough DB, McIntosh FA, Spanos C, Neelamegham S, Goldsmith HL, Simon SI., "Cooperativity between selectins and beta2-integrins define neutrophil capture and stable adhesion in shear flow" Ann Biomed Eng.32(9):1179-92, 2004 [ABSTRACT]
Neelamegham, S. "Transport features, reaction kinetics and receptor biomechanics controlling selectin and integrin mediated cell adhesion", Cell Communication and Adhesion, 11(1):35-50, 2004. [ABSTRACT]
Chandrasekaran EV, Lakhaman SS, Chawda R, Piskorz CF, Neelamegham S, Matta KL. "Identification of physiologically relevant substrates for cloned Gal: 3-O-sulfotransferases (Gal3STs): distinct high affinity of Gal3ST-2 and LS180 sulfotransferase for the globo H backbone, Gal3ST-3 for N-glycan multiterminal Galbeta1, 4GlcNAcbeta units and 6-sulfoGalbeta1, 4GlcNAcbeta, and Gal3ST-4 for the mucin core-2 trisaccharide", 279(11):10032-41, J. Biol. Chem., 2004. [ABSTRACT]
Shankaran H., and Neelamegham, S. "Hydrodynamic forces applied on intercellular bonds, soluble molecules and cell surface receptors", 86(1):576-588, Biophys J., 2004 [ABSTRACT].
Zhang, Y., and Neelamegham, S., "An analysis tool to quantify the efficiency of cell tethering and firm-adhesion in the parallel-plate flow chamber", 278(1-2): 305-317, Journal of Immunological Methods, 2003. [PDF]
Shankaran H, Alexandridis P, Neelamegham S., "Aspects of hydrodynamic shear regulating shear-induced platelet activation and self-association of von Willebrand factor in suspension.", 101(7): 2637-45, Blood. 2003 [ABSTRACT] [Commentary on this paper Inside Blood]
Zhang Y., Neelamegham, S., "PPLATE: A program for analysis of parallel plate flow chamber experimental data", 278(1-2): 319-321, Journal of Immunological Methods, 2003. [PDF]
Zhang, Y., and Neelamegham, S., "Estimating the efficiency of cell capture and arrest in flow chambers: Study of neutrophil binding via E-selectin and ICAM-1", 83(4):1934-52, Biophys. J., 2002. [ABSTRACT][Supplemental Data] [Response to letter to Editor].
Neelamegham, S. and Matta, K.L., "Liposomes bearing carbohydrates: Ligands for selectins", 199:175-91, Methods Mol Biol. 2002. [ABSTRACT]
Shankaran H., and Neelamegham, S. "Effect of Secondary Flow on Biological Experiments in the Cone-Plate Viscometer: Methods for estimating Collision Frequency, Wall Shear Stress and inter-particle Interactions", 38(4):275-304, Biorheology, 2001. [ABSTRACT]
Ahmed F., Alexandridis P., Shankaran, H., Neelamegham S., "The Ability of Poloxamers to Inhibit Platelet Aggregation Depends on Their Physicochemical Properties", 86(6),1532-9, Thrombosis and Homeostasis, 2001. [ABSTRACT]
Shankaran, H. and Neelamegham, S., "Non-Linear Flow Affects Hydrodynamic Forces and Neutrophil Adhesion Rates in Cone-Plate Viscometers" Biophysical J., 80(6):2631-2648, 2001. [ABSTRACT]
Ahmed, F. Alexandridis, P., Neelamegham, S., "Synthesis and Application of Fluorescein Labeled Pluronic Block Copolymers to Study Polymer-Surface Interactions", Langmuir, 17: 537-546, 2001. [ABSTRACT]
Neelamegham S., Taylor A.D., Shankaran, H., Smith, C.W., Simon, S.I., "Shear and Time-Dependent Changes in Mac-1, LFA-1, and ICAM-3 Binding Regulate Neutrophil Homotypic Adhesion", Journal of Immunology, 164(7): 3798-3805, 2000. [ABSTRACT]
Hentzen E.R., Neelamegham S., Kansas, G., McIntire, L.V., Smith, C.W., Simon, S.I., "Sequential binding of CD11a/CD18 and CD11b/CD18 define neutrophil capture and stable adhesion to ICAM-1", Blood, 95(3): 911-920, 2000. [ABSTRACT]
Hellums, J.D., Taylor, A.D., Neelamegham, S., Simon, S.I., "Rheology and molecular events in neutrophil aggregation", Journal of the Japanese Society of Biorheology, 12(1): 12-35, 1998.
Neelamegham, S., Taylor A.D., Burns, A.R., Smith, C.W., Simon, S.I., "Hydrodynamic shear reveals distinct roles for LFA-1 and Mac-1 in neutrophil adhesion to ICAM-1", Blood, 92(5): 1626-1638, 1998. [ABSTRACT]
*Neelamegham, S., *Konstantopolous, K., Burns, A.R., Hentzen, E., Kansas, G.S., Snapp, K.R., Berg, E.L., Hellums, J.D., Smith, C.W., McIntire, L.V., Simon, S.I., "Venous levels of shear induces neutrophil-platelet and neutrophil aggregation in blood via P-selectin and b2-integrin", Circulation, 98: 873-882, 1998. * Both authors are joint first authors. [ABSTRACT]
Simon, S.I. Neelamegham S., Taylor A.D., Smith C.W., "The multistep process of homotypic neutrophil aggregation: a review of the molecules and affects of hydrodynamics", Cell Adhesion and Communication, 6(2-3):263-76, 1998. [ABSTRACT]
Lynam E., Sklar L.A., Taylor A.D., Neelamegham S., Edwards, B.S., Smith, C.W., Simon, S.I., "Contributions of Mac-1 and LFA-1 in neutrophil adhesion to an ICAM-1 transfectant under hydrodynamic shear", Journal of Leukocyte Biology, 64(5):622-30, 1998. [ABSTRACT]
Neelamegham, S., Munn, L.L., Zygourakis, K., "A mathematical model for the kinetics of homotypic cellular aggregation under static conditions", Biophysical Journal, 72: 51-64, 1997. [ABSTRACT]
Neelamegham, S., Zygourakis, K., "A quantitative assay for intercellular aggregation", The Annals of Biomedical Engineering, 25 (1): 180-189, 1997. [ABSTRACT]
Neelamegham, S., Taylor, A.D., Hellums J.D., Dembo M., Smith C.W., Simon, S.I., "Modeling the reversible kinetics of neutrophil aggregation under hydrodynamic shear", Biophysical Journal, 72: 1527-1540, 1997. [ABSTRACT]
McIntyre, B.W., Woodside, D.G., Caruso, D.A., Wooten, D.K., Simon, S.I., Neelamegham, S., Revelle, J.K., Vanderslice, P., "Regulation of human lymphocyte coactivation with an a4 integrin antagonist peptide", Journal of Immunology, 158: 4180-4186, 1997. [ABSTRACT]
Tsang, Y.T.M., Neelamegham, S., Hu, Y., Burns, A.R., Berg, E.L. , Smith, C.W., Simon, S.I., "Synergy between L-selectin signaling and chemotactic activation during neutrophil adhesion and transmigration", Journal of Immunology, 159: 4566-4579, 1997. [ABSTRACT]
Neelamegham, S., Simon, S.I., McIntyre, B.W., Zygourakis, K., "Induction of homotypic lymphocyte aggregation: evidence for a novel activation state of the b1 Integrin.", Journal of Leukocyte Biology, 59: 872-882 , 1996. [ABSTRACT]
Taylor, A.D., Neelamegham, S., Hellums, J.D., Smith, C.W., Simon,
S.I.
"Molecular dynamics of the transition from L-selectin to beta
2-Integrin
dependent neutrophil adhesion under defined hydrodynamic shear",
Biophysical
Journal, 71: 3488-3500, 1996. [ABSTRACT]
Patents:
"Recovery of liquid from absorbent packaging material", Alexandridis,
P., and Neelamegham, S., U.S. patent 6,589,797, 2003 [Licensed to
Technicor,
Inc. Amherst, NY].
Courses Taught:
Biochemical Engineering, CE446/547
Transport Process II, CE318
Fluid Mechanics Laboratory, CE427
Heat and Mass Transfer Laboratory CE428
Cell and Molecular Bioengineering CE405/505