Bioengineering Laboratory
Department of Chemical Engineering
906 Furnas Hall
State University of New York at Buffalo
Buffalo, NY 14260-4200
Phone: (716) 645-2911 ext. 2220
Fax : (716) 645-3822
E-mail: neel@eng.buffalo.edu
|
Sriram Neelamegham Bioengineering Laboratory Department of Chemical Engineering 906 Furnas Hall State University of New York at Buffalo Buffalo, NY 14260-4200 Phone: (716) 645-2911 ext. 2220 Fax : (716) 645-3822 E-mail: neel@eng.buffalo.edu |
Professor, State University of New York at Buffalo, 2008-present
Associate Professor, State University of New York at Buffalo,
2003-2008
Assistant Professor, State University of New York at Buffalo, 1997-2003
Research Associate, Baylor College
of Medicine, Houston, 1995- 1997.
Ph.D., Chemical/Biomedical Engineering, Rice
University, Houston, 1996.
B.Tech., Chemical Engineering, Indian Institute of Technology, Delhi,
India, 1991.
· Research Interests
· Publications
· Courses
· Supplemental
Data
Research Interests:
Research in our laboratory lies
in the fields of Biomedical Engineering and Vascular Biology. The
underlying theme is to
apply
novel bioengineering techniques and quantitative methodologies in
combination
with fundamental biological principles, to elucidate the parameters and
mechanisms that regulate leukocyte and platelet function. The
importance
of the studies stem from the fact that ailments of the blood involving
these cell types account for a sizable fraction of inflammatory and
thrombotic
disorders. The focus of our work is on three families of adhesion
molecules
and their ligands expressed on blood cells and the vascular
endothelium.
These molecules are members of the selectin, integrin and Ig gene
superfamily.
Ongoing projects in the laboratory also examine the effects of fluid
shear
on the structure and function of plasma protein, von Willebrand Factor.
Some of the emphasis of
our
laboratory is on developing experimental and theoretical
bioengineering/
biophysical approaches to determine the features that regulate
leukocyte
and platelet adhesion. In particular, we are interested in
understanding
how mechanical forces applied on vascular cells due to fluid flow
modulate
cellular and biomolecular function. We are also interested in
developing "systems-based" experimental and theoretical approaches to
investigate biochemical reaction networks that relate to cellular
glycosylation and signal transduction. The long term goal is to develop
basic science understanding that can aid the development of novel
therapeutic strategies to combat
human inflammatory
and thrombotic diseases.
Dayananda K.M., Singh I., Mondal N., Neelamegham S. "von Willebrand factor self-association on platelet GpIb{alpha} under hydrodynamic shear: effect on shear-induced platelet activation.", Blood 116(19):3990-8, 2010. [ABSTRACT]. Commentary on this article [LINK]
Dayananda K.M., Gogia S., Neelamegham S. "E. coli derived Von Willebrand Factor-A2 domain FRET proteins that quantify ADAMTS13 activity", Analytical Biochemistry, 2010 (in press) [ABSTRACT]
Marathe D.D., Buffone A. Jr, Chandrasekaran E.V., Xue J., Locke R.D., Nasirikenari M., Lau J.T., Matta K.L., Neelamegham S., "Fluorinated per-acetylated GalNAc metabolically alters glycan structures on leukocyte PSGL-1 and reduces cell binding to selectins", Blood 115(6):1303-12, 2010. [ABSTRACT] Commentary on this article [LINK]Lim, K. H., Madabushi, S.R., Mann, J., Neelamegham,
S., Park, S. "Disulfide
trapping of
transient protein complexes on the yeast surface", Biotechnol Bioeng. 106(1):27-41, 2010. [ABSTRACT]
Liu G., Neelamegham S., "In silico Biochemical Reaction
Network Analysis (iBRENA): a
package for simulation and analysis of reaction networks" Bioinformatics
24(8):1109-11, 2008 [ABSTRACT]
[SOFTWARE DOWNLOAD
PAGE].
, "Differential regulation of neutrophil CD18 integrin function by di- and tri-valent cations: manganese vs. gadolinium", Ann Biomed Eng. 6(4):647-60, 2008 [ABSTRACT].
"Reversible
sialylation: synthesis of cytidine 5'-monophospho-N-acetylneuraminic
acid from cytidine 5'-monophosphate with a2,3-sialyl O-glycan-,
glycolipid-, and macromolecule-based donors yields diverse sialylated
products", Biochemistry,
47(1):320-30, 2008 [ABSTRACT].
Singh, I., Shankaran, H., Beauharnois, M.B.,
Xiao, Z., Alezxandridis, P., Neelamegham, S., "Solution structure of
human Von Willebrand factor studied using small angle neutron
scattering", J. Biol. Chem., 281(50):38266-275, 2006 [ABSTRACT].
Chandrasekaran, E.V., Xue, J., Neelamegham, S.,
Matta, K.L., "The pattern of glycosyl- and sulfotransferase activities
in cancer cell lines: a predictor of individual cancer-associated
distinct carbohydrate structures for the structural identification of
signature glycans", Carbohydrate Res. 341(8): 983-94, 2006 [ABSTRACT].
Xiao, Z., Goldsmith, H.L., McIntosh, F.A., Shankaran, H., Neelamegham, S., “P-selectin PSGL-1 bonds in neutrophil-platelet adhesion: Shear threshold and integrin-independent cell adhesion”, Biophys. J., 90(6):2221-34, 2006 [ABSTRACT].
Beauharnois, M.E., Lindquist, K.C., Marathe, D., Vanderslice, P., Xia, J., Matta, K.L., Neelamegham, S. “Affinity and Kinetics of Sialyl Lewis-X and Core-2 based Oligosaccharides Binding to L- and P-selectin ”, 44(27):9507-19, Biochemistry, 2005 [ABSTRACT].
Chandrasekaran, E.V. Xue, J., Xia, J., Chawda, R., Piskorz, C., Locke, R.D., Neelamegham, S., Matta, K.L. “Specificity analysis of sialyltransferases towards mucin core-2, Globo and related structures: Identification of the sialylation-sequence and the effects of sulfate, fucose, methyl and fluoro substituents of the carbohydrate chain in thebiosynthesis of selectin and siglec ligands, and novel sialylation by cloned alpha 2,3(O)sialyltransferase ”, 44(47):15619-35, Biochemistry, 2005 [ABSTRACT].
Liu, G., Swihart, M.T., Neelamegham, S., "Sensitivity, principal component and flux analysis applied to signal transduction: The case of epidermal growth factor mediated signaling", 21(7):1194-202, Bioinformatics, 2005 [ABSTRACT][Supplemental Data]
Neelamegham, S. "Transport features, reaction kinetics and receptor biomechanics controlling selectin and integrin mediated cell adhesion", Cell Communication and Adhesion, 11(1):35-50, 2004. [ABSTRACT]
Chandrasekaran EV, Lakhaman SS, Chawda R, Piskorz CF, Neelamegham S, Matta KL. "Identification of physiologically relevant substrates for cloned Gal: 3-O-sulfotransferases (Gal3STs): distinct high affinity of Gal3ST-2 and LS180 sulfotransferase for the globo H backbone, Gal3ST-3 for N-glycan multiterminal Galbeta1, 4GlcNAcbeta units and 6-sulfoGalbeta1, 4GlcNAcbeta, and Gal3ST-4 for the mucin core-2 trisaccharide", 279(11):10032-41, J. Biol. Chem., 2004. [ABSTRACT]
Shankaran H., and Neelamegham, S. "Hydrodynamic forces applied on intercellular bonds, soluble molecules and cell surface receptors", 86(1):576-588, Biophys J., 2004 [ABSTRACT].
Shankaran H, Alexandridis P, Neelamegham S., "Aspects of hydrodynamic shear regulating shear-induced platelet activation and self-association of von Willebrand factor in suspension.", 101(7): 2637-45, Blood. 2003 [ABSTRACT] [Commentary on this paper Inside Blood]
Zhang Y., Neelamegham, S., "PPLATE: A program for analysis of parallel plate flow chamber experimental data", 278(1-2): 319-321, Journal of Immunological Methods, 2003. [PDF]
Zhang, Y., and Neelamegham, S., "Estimating the efficiency of cell capture and arrest in flow chambers: Study of neutrophil binding via E-selectin and ICAM-1", 83(4):1934-52, Biophys. J., 2002. [ABSTRACT][Supplemental Data].
Neelamegham, S. and Matta, K.L., "Liposomes bearing carbohydrates: Ligands for selectins", 199:175-91, Methods Mol Biol. 2002. [ABSTRACT]
Selected Patents:
"Recovery of liquid from absorbent packaging material", Alexandridis,
P., and Neelamegham, S., U.S. patent 6,589,797, 2003 [Licensed to
Technicor,
Inc. Amherst, NY].
Courses Taught:
Biochemical Engineering, CE446/547
Bio Transport and Kinetics, CE500
Cell Biology-II, BMS505
Cell and Molecular Bioengineering, CE405/505
Transport Process II, CE318
Fluid Mechanics Laboratory, CE427
Heat and Mass Transfer Laboratory, CE428