Sriram Neelamegham Bioengineering Laboratory  Department of Chemical Engineering  906 Furnas Hall  State University of New York at Buffalo  Buffalo, NY 14260-4200  Phone: (716) 645-2911 ext. 2220  Fax : (716) 645-3822  E-mail: neel@eng.buffalo.edu

Professor, State University of New York at Buffalo, 2008-present
Associate Professor, State University of New York at Buffalo, 2003-2008
Assistant Professor, State University of New York at Buffalo, 1997-2003
Research Associate, Baylor College of Medicine, Houston, 1995- 1997.
Ph.D., Chemical/Biomedical Engineering, Rice University, Houston, 1996.
B.Tech., Chemical Engineering, Indian Institute of Technology, Delhi, India, 1991.
 

· RESEARCH OPENINGS
· Research Interests
· Publications
· Courses
· Supplemental Data

Research Interests:
       Research in our laboratory lies in the fields of Biomedical Engineering and Vascular Biology. The underlying theme is to apply novel bioengineering techniques and quantitative methodologies in combination with fundamental biological principles, to elucidate the parameters and mechanisms that regulate leukocyte and platelet function. The importance of the studies stem from the fact that ailments of the blood involving these cell types account for a sizable fraction of inflammatory and thrombotic disorders. The focus of our work is on three families of adhesion molecules and their ligands expressed on blood cells and the vascular endothelium. These molecules are members of the selectin, integrin and Ig gene superfamily. Ongoing projects in the laboratory also examine the effects of fluid shear on the structure and function of plasma protein, von Willebrand Factor.

        Some of the emphasis of our laboratory is on developing experimental and theoretical bioengineering/ biophysical approaches to determine the features that regulate leukocyte and platelet adhesion. In particular, we are interested in  understanding how mechanical forces applied on vascular cells due to fluid flow modulate cellular and biomolecular function. We are also interested in developing "systems-based" experimental and theoretical approaches to investigate biochemical reaction networks that relate to cellular glycosylation and signal transduction. The long term goal is to develop basic science understanding that can aid the development of novel therapeutic strategies to combat human inflammatory and thrombotic diseases.
 

Selected Publications:

Liu G., Neelamegham S., "In silico Biochemical Reaction Network Analysis (iBRENA): a package for simulation and analysis of reaction networks" Bioinformatics 24(8):1109-11, 2008 [ABSTRACT] [SOFTWARE DOWNLOAD PAGE].

Zhang Y, Hayenga HN, Sarantos MR, Simon SI, Neelamegham S., "Differential regulation of neutrophil CD18 integrin function by di- and tri-valent cations: manganese vs. gadolinium", Ann Biomed Eng. 6(4):647-60, 2008 [ABSTRACT].

Chandrasekaran EV, Xue J, Xia J, Locke RD, Matta KL, Neelamegham S. "Reversible sialylation: synthesis of cytidine 5'-monophospho-N-acetylneuraminic acid from cytidine 5'-monophosphate with a2,3-sialyl O-glycan-, glycolipid-, and macromolecule-based donors yields diverse sialylated products", Biochemistry, 47(1):320-30, 2008 [ABSTRACT].

Singh, I., Shankaran, H., Beauharnois, M.B., Xiao, Z., Alezxandridis, P., Neelamegham, S., "Solution structure of human Von Willebrand factor studied using small angle neutron scattering", J. Biol. Chem., 281(50):38266-275, 2006 [ABSTRACT].
 
Chandrasekaran, E.V., Xue, J., Neelamegham, S., Matta, K.L., "The pattern of glycosyl- and sulfotransferase activities in cancer cell lines: a predictor of individual cancer-associated distinct carbohydrate structures for the structural identification of signature glycans", Carbohydrate Res. 341(8): 983-94, 2006 [ABSTRACT].

Xiao, Z., Goldsmith, H.L., McIntosh, F.A., Shankaran, H., Neelamegham, S., “P-selectin PSGL-1 bonds in neutrophil-platelet adhesion: Shear threshold and integrin-independent cell adhesion”, Biophys. J., 90(6):2221-34, 2006 [ABSTRACT].

Beauharnois, M.E., Lindquist, K.C., Marathe, D., Vanderslice, P., Xia, J., Matta, K.L., Neelamegham, S. “Affinity and Kinetics of Sialyl Lewis-X and Core-2 based Oligosaccharides Binding to L- and P-selectin ”, 44(27):9507-19, Biochemistry, 2005 [ABSTRACT].

Chandrasekaran, E.V. Xue, J., Xia, J., Chawda, R., Piskorz, C., Locke, R.D., Neelamegham, S., Matta, K.L. “Specificity analysis of sialyltransferases towards mucin core-2, Globo and related structures: Identification of the sialylation-sequence and the effects of sulfate, fucose, methyl and fluoro substituents of the carbohydrate chain in thebiosynthesis of selectin and siglec ligands, and novel sialylation by cloned alpha 2,3(O)sialyltransferase ”, 44(47):15619-35, Biochemistry, 2005 [ABSTRACT].

Liu, G., Swihart, M.T., Neelamegham, S., "Sensitivity, principal component and flux analysis applied to signal transduction: The case of epidermal growth factor mediated signaling", 21(7):1194-202, Bioinformatics, 2005 [ABSTRACT][Supplemental Data]

Neelamegham, S. "Transport features, reaction kinetics and receptor biomechanics controlling selectin and integrin mediated cell adhesion", Cell Communication and Adhesion, 11(1):35-50, 2004. [ABSTRACT]

Chandrasekaran EV, Lakhaman SS, Chawda R, Piskorz CF, Neelamegham S, Matta KL. "Identification of physiologically relevant substrates for cloned Gal: 3-O-sulfotransferases (Gal3STs): distinct high affinity of Gal3ST-2 and LS180 sulfotransferase for the globo H backbone, Gal3ST-3 for N-glycan multiterminal Galbeta1, 4GlcNAcbeta units and 6-sulfoGalbeta1, 4GlcNAcbeta, and Gal3ST-4 for the mucin core-2 trisaccharide", 279(11):10032-41, J. Biol. Chem., 2004. [ABSTRACT]

Shankaran H., and Neelamegham, S. "Hydrodynamic forces applied on intercellular bonds, soluble molecules and cell surface receptors", 86(1):576-588, Biophys J., 2004 [ABSTRACT].

Shankaran H, Alexandridis P, Neelamegham S., "Aspects of hydrodynamic shear regulating shear-induced platelet activation and self-association of von Willebrand factor in suspension.", 101(7): 2637-45, Blood. 2003 [ABSTRACT] [Commentary on this paper Inside Blood]

Zhang Y., Neelamegham, S., "PPLATE: A program for analysis of parallel plate flow chamber experimental data", 278(1-2): 319-321, Journal of Immunological Methods, 2003. [PDF]

Zhang, Y., and Neelamegham, S., "Estimating the efficiency of cell capture and arrest in flow chambers: Study of neutrophil binding via E-selectin and ICAM-1", 83(4):1934-52, Biophys. J., 2002. [ABSTRACT][Supplemental Data].

Neelamegham, S. and Matta, K.L., "Liposomes bearing carbohydrates: Ligands for selectins", 199:175-91, Methods Mol Biol. 2002. [ABSTRACT]

 Selected Patents:
"Recovery of liquid from absorbent packaging material", Alexandridis, P., and Neelamegham, S., U.S. patent 6,589,797, 2003 [Licensed to Technicor, Inc. Amherst, NY].

Courses Taught:
Biochemical Engineering, CE446/547
Bio Transport and Kinetics, CE500
Cell Biology-II, BMS505
Cell and Molecular Bioengineering, CE405/505
Transport Process II, CE318
Fluid Mechanics Laboratory, CE427
Heat and Mass Transfer Laboratory, CE428