Mattheos Koffas Publications

Publications and Patents
Literature publications, U.S. patents and other submissions.

Peer-reviewed Publications

Leonard, E.; Lim, K.-H.; Shaw, P.-N.; Koffas, M.A.G.; “Engineering central metabolic Pathways for high-level flavonoid production in Escherichia coli”, Applied and Environmental Microbiology 2007 Apr. 27; 73(12): 3877-3886.

Chemler, J.; Yan, Y.; Leonard, E.; Koffas, M.A.G.; “Combinatorial mutasynthesis of flavonoid analogues from acrylic acids in microorganisms”, Organic Letters 2007 May 10; 9(10): 1855-8

Chemler, J.; Yan, Y.; Koffas, M.A.G. “Biosynthesis of isoprenoids, polyunsaturated fatty acids and flavonoids in Saccharomyces cerevisiae”, Microbial Cell Factories 2006 5(20):

Leonard, E.; Yan, Y.; Koffas, M.A.G. (2006) “Functional expression of a P450 flavonoid hydroxylase for the biosynthesis of plant-specific hydroxylated flavonols in Escherichia coli”, Metabolic Engineering 8(2): 172-181.

Leonard, E.; Yan, Y.; Lim, K.H.; Koffas, M.A.G. (2005) “Investigation of two distinct flavone synthases for plant-specific flavone biosynthesis in Saccharomyces cerevisiae”, Applied and Environmental Microbiology, 71(12): 8241-8248.

Yan, Y.; Kohli, A.; Koffas, M.A.G.; (2005); "Biosynthesis of natural flavanones in Saccharomyces cerevisiae”, Applied and Environmental Microbiology, 71(9): 5610-5613.

Leonard, E.; Chemler, J.; Lim K. H.; Koffas, M.A.G. (2006); "Expression of a soluble flavone synthase allows the biosynthesis of phytoestrogen derivatives in Escherichia coli", Applied Microbiology and Biotechnology, 70(1):85-91.

Yan, Y.; Chemler, J.; Huang, L.; Martens, S.; Koffas, M.A.G. (2005). "Metabolic Engineering of anthocyanin biosynthesis in Escherichia coli", Applied and Environmental Microbiology. 71(7): 3617- 3623.

Koffas, M.A.G. and DelCardayre, S (2005). "Evolutionary Metabolic Engineering" Metabolic Engineering 7(1): 1-3.

Koffas, M.A.G. and Stephanopoulos, G. (2005); "Strain improvement by metabolic engineering: lysine production as a case study for systems biology", Current Opinion in Biotechnology 16(3):361-366.

Koffas, M.A.G.; Jung, G.Y. and Stephanopoulos, G. (2003) “Engineering metabolism and product formation in Corynebacterium glutamicum by coordinated gene overexpression”, Metabolic Engineering, 5 (1), 32-41.

Koffas, M.A.G.; Jung, G.Y.; Aon, J.C. and Stephanopoulos, G. (2002) “Effect of pyruvate carboxylase overexpression on the physiology of Corynebacterium glutamicum”, Applied and Environmental Microbiology, 68 (11), 5422-5428.

Koffas, M.A.G.; Roberge, C.M.; Lee, K. and Stephanopoulos, G. (1999) “Metabolic Engineering”, Annual Review of Biomedical Engineering, 1, 535-557.

Koffas, M.A.G.; Ramamoorthi, R.; Pine, W.A.; Sinskey, A.J. and Stephanopoulos, G. (1998) “Sequence of the Corynebacterium glutamicum pyruvate carboxylase gene”, Applied Microbiology and Biotechnology, 50 (3), 346-352.

Book Chapters

Fowler, Z.; Leonard, E. and Koffas, M.A.G. 2007. Biosynthesis of essential building blocks. In C. Smolke (ed.), Metabolic Engineering [in press] CRC Press

Fowler, Z. and Koffas, M.A.G. 2007. Microbial biosynthesis of fine chemicals. In C. Smolke (ed.), Metabolic Engineering [in press] CRC Press

Chemler, J.; Leonard, E. and Koffas, M.A.G. 2007. Flavonoid biotranformations in microorganisms. In K. Gould, K. Davies and C. Winefield (ed.)Anthocyanins: biosynthesis, function and application [in press] Springer

Leonard, E.; Fowler, Z. and Koffas, M.A.G. 2007. Metabolic Engineering, p. 301-359. In M. Al-Rubeai and M. Fusseneger (ed.), Systems Biology, vol. 5. 645 Springer, London.

Electronic Submissions

Leonard. E.; Kohara, Y.; Shin-i, T.; Sugano, S.; Thierry-Mieg, D.; Suzuki, Y. and Koffas, M.A.G; Caenorhabditis elegans biotin protein ligase, holocarboxylase synthetase (62.5 kD) alternative variant d (bpl-1), complete mRNA, NCBI Nucleotides database.

Leonard. E.; Kohara, Y.; Shin-i, T.; Sugano, S.; Thierry-Mieg, D.; Suzuki, Y. and Koffas, M.A.G; Caenorhabditis elegans biotin protein ligase, holocarboxylase synthetase (62.5 kD) alternative variant b (bpl-1), complete mRNA, NCBI Nucleotides database.

Leonard. E.; Kohara, Y.; Shin-i, T.; Sugano, S.; Thierry-Mieg, D. and Koffas, M.A.G; Caenorhabditis elegans biotin protein ligase, holocarboxylase synthetase (70.9 kD) alternative variant c (bpl-1), complete mRNA, NCBI Nucleotides database.

Yan, Y. and Koffas, M.A.G. Lilium hybrid cv. 'Star Gazer' dihydroxyflavonol 4-reductase (DFR), NCBI Nucleotides database.

U.S. Patents

Koffas, M.A.G.; Leonard, E.; Yan, Y.; Chemler, J.; “Production of flavonoids by recombinant microorganisms”. Patent Application 11/178,912, University at Buffalo, the State University of New York.

Koffas, M.A.G.; Odom, J.M.; Shenzle, A.; “Production of feed using a denitrifying methanotrophic bacterial strain”, US6958222(B2), DuPont De Nemours CR&D.

Koffas, M.A.G.; Odom, J.M.; Shenzle, A.; “High growth methanotrophic bacterial strain Methylomonas 16a” WO0220728, DuPont De Nemours CR&D.

Koffas, M.A.G.; Norton, K.; Odom, J.M.; Ye, R.W.; “Methanotrophic carbon metabolism pathway genes and enzymes” WO0220796, DuPont De Nemours CR&D.

Koffas, M.A.G.; Odom, J.M.; Wang, S.; Wang, T.; Ye, R.W.; “Genes encoding polysaccharide production” WO0220797, DuPont De Nemours CR&D.

Cheng, Q.; Koffas, M.A.G.; Norton, K.; Odom, J.M.; Picataggio, S.K.; Rouviere, P.E.; Schenzle, A.; Tomb, J.-F.;“Genes involved in isoprenoid compound production” WO0220733, DuPont De Nemours CR&D.

DiCosimo, D.; Koffas, M.A.G.; Wang,, S.; “Production of cyclic terpenoids” WO0220815, DuPont De Nemours CR&D.

Brzostowicz, P.C.; Cheng, Q.; DiCosimo, D.J.; Koffas, M.A.G.; Miller, E.S.; Odom, J.M.; Picattagio, S.K.; Rouviere, P.E.; “Carotenoid production from a single carbon substrate” WO0218617, DuPont De Nemours CR&D.