Week	Date	Lecture topics	Readings	Lab	Lab Report	Lecturer
1	Wed Jan 19	Background genetic	Ch. 1,2,	DNA isolation: sample prep	No	MAC
	Fri. Jan. 21	Sources of Variation		DNA isolation: extraction		MAC
2	Wed, Jan 26	Basic Molecular Techniques		DNA isolation: precipitation, resuspend, quantification and RNase		MAC
	Fri. Jan. 28	Survey of Techniques (Allozymes)	Ch. 3	Allozymes: sample prep, run gel, staining	Yes, due Feb 11	MAC
3	Wed Feb. 2.	Survey of Techniques (AFLP, VNTR, RAPDs)	Ch. 3	Allozymes: run gel, staining		MAC
	Fri. Feb. 4	Survey of Techinques (SSR, ISSR, RFLP of nuclear, mt and cp DNA markers)	Ch. 3	Allozymes: data analysis		MAC, Computer lab
4	Wed. Feb. 9	Survey of Techniques (Sequence analysis)	Ch. 3	AFLP: digestion and ligation	Yes, due Mar 1	MAC
	Fri. Feb. 11	Phylogenetic reconstruction	Ch. 4, 8	AFLP: set up PCR, pour gels		MAC
5	Wed, Feb 16	Phylogenetic reconstruction		AFLP: run Li-cor		MAC
	Fri. Feb. 18	Population structure I: basic principles	Ch. 5	SSR: digestion and size fraction	Yes, due Apr 14	GY,
6	Wed. Feb 23	Population structure I (cont.)		AFLP: data analysis		GY,Computer lab
	Fri. Feb. 25	Population structure II: population subdivision	Ch. 6	SSR: DNA purification from gel, quantification		GY
7	Wed. Mar 1	Population structure II (cont.)		SSR: ligation, prep competent E. coli cells (Feb. 17)		GY
	Fri. Mar 3	EXAM 1		NO LAB

 
 

Week	Date	Lecture topics	Readings	Lab	Lab Report	Lecturer
8	Wed. Mar 8	NO CLASS		SPRING BREAK
	Fri. Mar 10	NO CLASS		SPRING BREAK
9	Wed. Mar 15	Population structure III: gene flow	Ch. 6	SSR: transformation PCR-RFLP: mtDNA and cpDNA PCR	Yes, due Mar 31	GY
	Fri Mar 17.	Population structure III (cont.)		SSR: Plate lifts, pre-block PCR-RFLP: check PCR product quality		GY
10	Wed Mar 22.	Population structure IV: parentage and kinship	Ch. 6	SSR: Hybridization PCR-RFLP: digestion PCR product and score		GY
	Fri Mar 24.	Population structure IV (cont.)		SSR: Pick positive clones and grow PCR-RFLP -- analysis of mtDNA and cpDNA data		GY: Computer lab
11	Wed. Mar 29	Population structure V: Phylogeography	Ch. 6	SSR: Miniprep for sequencing		GY
	Fri. Mar 31	Population structure V (cont.)		SSR: design primers, setup SSR, make PAGE gel		GY
12	Wed. Apr 5	Conservation genetics I	Ch. 9	SSR: Run out PCR product, load Li-Cor ITS: set up PCR	Yes, due Apr 26	GY: Computer lab
	Fri. Apr. 7	Conservation genetics I (cont.)		SSR: data analysis		GY
13	Wed. Apr. 12	Conservation genetics II	Ch. 9	ITS: check PCR product quality, prep sequencing reaction, pour gel		GY

 
 

Week	Date	Lecture topics	Readings	Lab	Lab Report	Lecturer
	Fri. Apr. 14	Conservation genetics II (cont.)		ITS: run Li-cor		GY
14	Wed. Apr. 19	Conservation genetics II (cont.)		ITS: sequence data analysis		GY: Computer lab
	Fri. Apr. 21	EXAM 2		No LAB
15	Wed Apr. 26.	Oral Presentations		Oral Presentations
	Fri. Apr. 28	Field trip		Field trip